English: Differences in cysteine and serineproteolysismechanisms.
The protease (black) performs a nucleophilic attack on peptide substrate (red) to form a tetrahedral intermediate. This breaks down by ejection of the first product, the substrate C-terminus, to form the acyl-enzyme intermediate. Water replaces the first product and hydrolysis occurs via a second tetrahedral intermediate to regenerate free enzyme. Indicated differences are (a) the deprotonated cysteine, (b) aspartate (grey) not present in all cysteine proteases, (c) concerted deprotonation of serine, (d) aspartate hydrogen bonding, (e) amide protonation of the first leaving group, (f) alcohol protonation of the serine leaving group.
láti pín pẹ̀lú ẹlòmíràn – láti ṣàwòkọ, pínkiri àti ṣàgbéká iṣẹ́ náà
láti túndàpọ̀ – láti mulò mọ́ iṣẹ́ míràn
Lábẹ́ àwọn àdéhùn wọ̀nyí:
ìdárúkọ – Ẹ gbọdọ̀ ṣe ọ̀wọ̀ tó yẹ, pèsè ìjápọ̀ sí ìwé-àṣe, kí ẹ sì sọ bóyá ìyípadà wáyé. Ẹ le ṣe èyí lórísi ọ̀nà tó bojúmu, sùgbọ́n tí kò ní dà bii pé oníìwé-àṣe fọwọ́ sí yín tàbí lílò yín.
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