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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1]
Ọjọ́ọdún
Orísun Iṣẹ́ onítọ̀hún
Olùdá Thomas Shafee
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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  1. Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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RNA sequencing Èdè Gẹ̀ẹ́sì

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5 Oṣù Ọ̀wàrà 2016

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lọ́wọ́12:20, 13 Oṣù Èrèlé 2017Àwòrán kékeré fún ní 12:20, 13 Oṣù Èrèlé 20171,020 × 803 (246 KB)Evolution and evolvabilitytext as paths to avoid artefacts
12:19, 13 Oṣù Èrèlé 2017Àwòrán kékeré fún ní 12:19, 13 Oṣù Èrèlé 20171,020 × 803 (90 KB)Evolution and evolvabilitytext as paths to avoid artefacts
10:48, 13 Oṣù Èrèlé 2017Àwòrán kékeré fún ní 10:48, 13 Oṣù Èrèlé 20171,020 × 803 (88 KB)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
08:41, 10 Oṣù Èrèlé 2017Àwòrán kékeré fún ní 08:41, 10 Oṣù Èrèlé 20171,020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription
05:08, 5 Oṣù Ọ̀wàrà 2016Àwòrán kékeré fún ní 05:08, 5 Oṣù Ọ̀wàrà 20161,020 × 803 (85 KB)Evolution and evolvabilityUser created page with UploadWizard

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