Fáìlì:Summary of SAGE.svg
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Àkótán
| Ìjúwe |
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1] |
| Ọjọ́ọdún | |
| Orísun | Iṣẹ́ onítọ̀hún |
| Olùdá | Thomas Shafee |
| Àwọn àtẹ̀jáde míràn |
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Ìwé àṣẹ
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- Ẹ ní ààyè:
- láti pín pẹ̀lú ẹlòmíràn – láti ṣàwòkọ, pínkiri àti ṣàgbéká iṣẹ́ náà
- láti túndàpọ̀ – láti mulò mọ́ iṣẹ́ míràn
- Lábẹ́ àwọn àdéhùn wọ̀nyí:
- ìdárúkọ – Ẹ gbọdọ̀ ṣe ọ̀wọ̀ tó yẹ, pèsè ìjápọ̀ sí ìwé-àṣe, kí ẹ sì sọ bóyá ìyípadà wáyé. Ẹ le ṣe èyí lórísi ọ̀nà tó bojúmu, sùgbọ́n tí kò ní dà bii pé oníìwé-àṣe fọwọ́ sí yín tàbí lílò yín.
- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
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Ẹ kan kliki lórí ọjọ́ọdún/àkókò kan láti wo fáìlì ọ̀ún bó ṣe hàn ní àkókò na.
| Ọjọ́ọdún/Àkókò | Àwòrán kékeré | Àwọn ìwọ̀n | Oníṣe | Àríwí | |
|---|---|---|---|---|---|
| lọ́wọ́ | 12:21, 13 Oṣù Èrèlé 2017 | | 938 × 756 (180 KB) | Evolution and evolvability | text as paths to avoid artefacts |
| 10:48, 13 Oṣù Èrèlé 2017 |  | 938 × 756 (104 KB) | Evolution and evolvability | update sequence text to 'sans-serif' open font for more robust rendering | |
| 05:27, 7 Oṣù Ọ̀wàrà 2016 |  | 938 × 756 (104 KB) | Evolution and evolvability | ce | |
| 05:23, 7 Oṣù Ọ̀wàrà 2016 |  | 938 × 756 (102 KB) | Evolution and evolvability | ce | |
| 05:16, 7 Oṣù Ọ̀wàrà 2016 |  | 938 × 756 (104 KB) | Evolution and evolvability | digitag-->tag | |
| 05:08, 5 Oṣù Ọ̀wàrà 2016 |  | 1,020 × 821 (106 KB) | Evolution and evolvability | User created page with UploadWizard |
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